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ptk88 gfp anillin plasmid  (Addgene inc)


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    Addgene inc ptk88 gfp anillin plasmid
    Ptk88 Gfp Anillin Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ptk88+gfp+anillin+plasmid/pTK88_GFP-Anillin+(Plasmid+%2346354)/10__1091_slash_mbc__e21___02___0072-190-6-8
    Average 92 stars, based on 2 article reviews
    ptk88 gfp anillin plasmid - by Bioz Stars, 2026-10
    92/100 stars

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    Related Articles

    Transfection:

    Article Title: Substratum stiffness signals through integrin-linked kinase and β1-integrin to regulate midbody proteins and abscission during EMT
    Article Snippet: Abscission is the final stage of cytokinesis during which the parent cell physically separates to yield two identical daughters.. Failure of abscission results in multinucleation (MNC), a sign of genomic instability and a precursor to aneuploidy, enabling characteristics of neoplastic progression.. Induction of epithelial-mesenchymal transition (EMT) causes MNC in mammary epithelial cells cultured on stiff microenvironments that have mechanical properties similar to those found in breast tumors, but not on soft microenvironments reminiscent of the normal mammary gland.

    Article Title: Substratum stiffness signals through integrin-linked kinase and β1-integrin to regulate midbody proteins and abscission during EMT
    Article Snippet: Stable clones were selected using puromycin (5 μg/ml; Sigma). .. NMuMG cells were transfected with the pTK88_GFP-Anillin plasmid (Addgene plasmid # 46354), the scramble-shRNA plasmid (shScr, Addgene plasmid #1864), the shAnillin plasmid (Sigma), or the shβ1 plasmid (Sigma) using FuGENE HD Transfection Reagent (Promega). ..

    Plasmid Preparation:

    Article Title: Substratum stiffness signals through integrin-linked kinase and β1-integrin to regulate midbody proteins and abscission during EMT
    Article Snippet: Abscission is the final stage of cytokinesis during which the parent cell physically separates to yield two identical daughters.. Failure of abscission results in multinucleation (MNC), a sign of genomic instability and a precursor to aneuploidy, enabling characteristics of neoplastic progression.. Induction of epithelial-mesenchymal transition (EMT) causes MNC in mammary epithelial cells cultured on stiff microenvironments that have mechanical properties similar to those found in breast tumors, but not on soft microenvironments reminiscent of the normal mammary gland.

    Article Title: Substratum stiffness signals through integrin-linked kinase and β1-integrin to regulate midbody proteins and abscission during EMT
    Article Snippet: Stable clones were selected using puromycin (5 μg/ml; Sigma). .. NMuMG cells were transfected with the pTK88_GFP-Anillin plasmid (Addgene plasmid # 46354), the scramble-shRNA plasmid (shScr, Addgene plasmid #1864), the shAnillin plasmid (Sigma), or the shβ1 plasmid (Sigma) using FuGENE HD Transfection Reagent (Promega). ..



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    ptk88 gfp anillin plasmid  (Addgene inc)
    92
    Addgene inc ptk88 gfp anillin plasmid
    Ptk88 Gfp Anillin Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ptk88+gfp+anillin+plasmid/pTK88_GFP-Anillin+(Plasmid+%2346354)/10__1091_slash_mbc__e21___02___0072-190-6-8
    Average 92 stars, based on 1 article reviews
    ptk88 gfp anillin plasmid - by Bioz Stars, 2026-10
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    nmumg cells  (Addgene inc)
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    Inducing EMT by exposing cells to TGFβ or ectopically expressing Snail leads to MNC through septin-6. (A) qRT-PCR analysis for Snai1 in <t>NMuMG</t> mouse mammary epithelial cells treated with or without TGFβ. (B) Phase-contrast and fluorescence images and (C) quantification of MNC in cells treated with or without TGFβ. (D) qRT-PCR analysis for Snai1 in cells ectopically <t>expressing</t> <t>GFP</t> or Snail. (E) Phase-contrast and fluorescence images and (F) quantification of MNC in cells ectopically expressing GFP or Snail. (G) qRT-PCR analysis for Sept6 in NMuMG cells ectopically expressing GFP or Snail. (H) qRT-PCR analysis for Sept6 in cells ectopically expressing GFP or septin-6. (I) Phase-contrast and fluorescence images and (J) quantification of MNC in cells ectopically expressing GFP or septin-6. (K) qRT-PCR analysis for Sept6 in cells ectopically expressing shCntl or shSeptin-6. (L) Phase-contrast and fluorescence images and (M) quantification of MNC in cells ectopically expressing shCntl or shSeptin-6 treated with or without TGFβ. Shown are mean ± SD of n = 3–4 independent experiments. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001 using two-sided Welch’s t test (A, D, G, H, K), two-sided Student’s t test (C, F, J), or two-way Anova with Tukey’s post-hoc test (M). Scale bars, 25 μm.
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    Average 92 stars, based on 1 article reviews
    nmumg cells - by Bioz Stars, 2026-10
    92/100 stars
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    ptk88 gfp anillin retroviral plasmid  (Addgene inc)
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    Inducing EMT by exposing cells to TGFβ or ectopically expressing Snail leads to MNC through septin-6. (A) qRT-PCR analysis for Snai1 in <t>NMuMG</t> mouse mammary epithelial cells treated with or without TGFβ. (B) Phase-contrast and fluorescence images and (C) quantification of MNC in cells treated with or without TGFβ. (D) qRT-PCR analysis for Snai1 in cells ectopically <t>expressing</t> <t>GFP</t> or Snail. (E) Phase-contrast and fluorescence images and (F) quantification of MNC in cells ectopically expressing GFP or Snail. (G) qRT-PCR analysis for Sept6 in NMuMG cells ectopically expressing GFP or Snail. (H) qRT-PCR analysis for Sept6 in cells ectopically expressing GFP or septin-6. (I) Phase-contrast and fluorescence images and (J) quantification of MNC in cells ectopically expressing GFP or septin-6. (K) qRT-PCR analysis for Sept6 in cells ectopically expressing shCntl or shSeptin-6. (L) Phase-contrast and fluorescence images and (M) quantification of MNC in cells ectopically expressing shCntl or shSeptin-6 treated with or without TGFβ. Shown are mean ± SD of n = 3–4 independent experiments. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001 using two-sided Welch’s t test (A, D, G, H, K), two-sided Student’s t test (C, F, J), or two-way Anova with Tukey’s post-hoc test (M). Scale bars, 25 μm.
    Ptk88 Gfp Anillin Retroviral Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Inducing EMT by exposing cells to TGFβ or ectopically expressing Snail leads to MNC through septin-6. (A) qRT-PCR analysis for Snai1 in NMuMG mouse mammary epithelial cells treated with or without TGFβ. (B) Phase-contrast and fluorescence images and (C) quantification of MNC in cells treated with or without TGFβ. (D) qRT-PCR analysis for Snai1 in cells ectopically expressing GFP or Snail. (E) Phase-contrast and fluorescence images and (F) quantification of MNC in cells ectopically expressing GFP or Snail. (G) qRT-PCR analysis for Sept6 in NMuMG cells ectopically expressing GFP or Snail. (H) qRT-PCR analysis for Sept6 in cells ectopically expressing GFP or septin-6. (I) Phase-contrast and fluorescence images and (J) quantification of MNC in cells ectopically expressing GFP or septin-6. (K) qRT-PCR analysis for Sept6 in cells ectopically expressing shCntl or shSeptin-6. (L) Phase-contrast and fluorescence images and (M) quantification of MNC in cells ectopically expressing shCntl or shSeptin-6 treated with or without TGFβ. Shown are mean ± SD of n = 3–4 independent experiments. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001 using two-sided Welch’s t test (A, D, G, H, K), two-sided Student’s t test (C, F, J), or two-way Anova with Tukey’s post-hoc test (M). Scale bars, 25 μm.

    Journal: Molecular Biology of the Cell

    Article Title: Substratum stiffness signals through integrin-linked kinase and β1-integrin to regulate midbody proteins and abscission during EMT

    doi: 10.1091/mbc.E21-02-0072

    Figure Lengend Snippet: Inducing EMT by exposing cells to TGFβ or ectopically expressing Snail leads to MNC through septin-6. (A) qRT-PCR analysis for Snai1 in NMuMG mouse mammary epithelial cells treated with or without TGFβ. (B) Phase-contrast and fluorescence images and (C) quantification of MNC in cells treated with or without TGFβ. (D) qRT-PCR analysis for Snai1 in cells ectopically expressing GFP or Snail. (E) Phase-contrast and fluorescence images and (F) quantification of MNC in cells ectopically expressing GFP or Snail. (G) qRT-PCR analysis for Sept6 in NMuMG cells ectopically expressing GFP or Snail. (H) qRT-PCR analysis for Sept6 in cells ectopically expressing GFP or septin-6. (I) Phase-contrast and fluorescence images and (J) quantification of MNC in cells ectopically expressing GFP or septin-6. (K) qRT-PCR analysis for Sept6 in cells ectopically expressing shCntl or shSeptin-6. (L) Phase-contrast and fluorescence images and (M) quantification of MNC in cells ectopically expressing shCntl or shSeptin-6 treated with or without TGFβ. Shown are mean ± SD of n = 3–4 independent experiments. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001 using two-sided Welch’s t test (A, D, G, H, K), two-sided Student’s t test (C, F, J), or two-way Anova with Tukey’s post-hoc test (M). Scale bars, 25 μm.

    Article Snippet: NMuMG cells were transfected with the pTK88_GFP-Anillin plasmid (Addgene plasmid # 46354), the scramble-shRNA plasmid (shScr, Addgene plasmid #1864), the shAnillin plasmid (Sigma), or the shβ1 plasmid (Sigma) using FuGENE HD Transfection Reagent (Promega).

    Techniques: Expressing, Quantitative RT-PCR, Fluorescence

    Snail and substratum stiffness increase the expression of midbody proteins. (A) Schematic of the abscission machinery. (B) qRT-PCR analysis for Sept6 in NMuMG mouse mammary epithelial cells cultured on soft or stiff substrata and ectopically expressing GFP or Snail. (C) qRT-PCR analysis for Anln (anillin) and Kif23 (Mklp1) in cells ectopically expressing GFP or Snail. (D) Immunoblotting analysis for anillin and Mklp1 in cells ectopically expressing GFP or Snail. (E) qRT-PCR analysis for Anln and Kif23 in cells cultured on soft or stiff substrata. (F) qRT-PCR analysis for Anln and Kif23 in cells cultured on soft or stiff substrata and ectopically expressing GFP or Snail. Shown are mean ± SD of n = 3-4 independent experiments. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001 using two-way ANOVA with Tukey’s post-hoc test (B, F) or two-sided Welch’s t test (C, E).

    Journal: Molecular Biology of the Cell

    Article Title: Substratum stiffness signals through integrin-linked kinase and β1-integrin to regulate midbody proteins and abscission during EMT

    doi: 10.1091/mbc.E21-02-0072

    Figure Lengend Snippet: Snail and substratum stiffness increase the expression of midbody proteins. (A) Schematic of the abscission machinery. (B) qRT-PCR analysis for Sept6 in NMuMG mouse mammary epithelial cells cultured on soft or stiff substrata and ectopically expressing GFP or Snail. (C) qRT-PCR analysis for Anln (anillin) and Kif23 (Mklp1) in cells ectopically expressing GFP or Snail. (D) Immunoblotting analysis for anillin and Mklp1 in cells ectopically expressing GFP or Snail. (E) qRT-PCR analysis for Anln and Kif23 in cells cultured on soft or stiff substrata. (F) qRT-PCR analysis for Anln and Kif23 in cells cultured on soft or stiff substrata and ectopically expressing GFP or Snail. Shown are mean ± SD of n = 3-4 independent experiments. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001 using two-way ANOVA with Tukey’s post-hoc test (B, F) or two-sided Welch’s t test (C, E).

    Article Snippet: NMuMG cells were transfected with the pTK88_GFP-Anillin plasmid (Addgene plasmid # 46354), the scramble-shRNA plasmid (shScr, Addgene plasmid #1864), the shAnillin plasmid (Sigma), or the shβ1 plasmid (Sigma) using FuGENE HD Transfection Reagent (Promega).

    Techniques: Expressing, Quantitative RT-PCR, Cell Culture, Western Blot

    A soft microenvironment protects against MNC even in cells that ectopically express septin-6 or Mklp1. (A) Phase-contrast and fluorescence images of NMuMG mouse mammary epithelial cells ectopically expressing GFP or septin-6 and cultured on soft or stiff substrata. Red, E-cadherin; white, nuclei. Quantification of MNC in cells ectopically expressing (B) septin-6 or (C) Mklp1 cultured on soft or stiff substrata. Shown are mean ± SD of n = 3 independent experiments. ** P < 0.01, **** P < 0.0001 using two-way ANOVA with Tukey’s post-hoc test. Scale bars, 10 μm.

    Journal: Molecular Biology of the Cell

    Article Title: Substratum stiffness signals through integrin-linked kinase and β1-integrin to regulate midbody proteins and abscission during EMT

    doi: 10.1091/mbc.E21-02-0072

    Figure Lengend Snippet: A soft microenvironment protects against MNC even in cells that ectopically express septin-6 or Mklp1. (A) Phase-contrast and fluorescence images of NMuMG mouse mammary epithelial cells ectopically expressing GFP or septin-6 and cultured on soft or stiff substrata. Red, E-cadherin; white, nuclei. Quantification of MNC in cells ectopically expressing (B) septin-6 or (C) Mklp1 cultured on soft or stiff substrata. Shown are mean ± SD of n = 3 independent experiments. ** P < 0.01, **** P < 0.0001 using two-way ANOVA with Tukey’s post-hoc test. Scale bars, 10 μm.

    Article Snippet: NMuMG cells were transfected with the pTK88_GFP-Anillin plasmid (Addgene plasmid # 46354), the scramble-shRNA plasmid (shScr, Addgene plasmid #1864), the shAnillin plasmid (Sigma), or the shβ1 plasmid (Sigma) using FuGENE HD Transfection Reagent (Promega).

    Techniques: Fluorescence, Expressing, Cell Culture